Below please start out the ninth of the weekly lab essayzes. All 10 points of the quiz volition come from this list of questions.
1.Describe in elaborate the assay you will do this week on the cellular fractions from last week. (5 points)
2.Fill in the following table so that all tubes have a final volume of 60 μl: (5 points)
Assay Tube #Desired [BSA](mg/ml)Volume of 1 mg/mlBSA (μl)Volume of phosphate buffer (μl)
1 = waste0060
20.2
30.4
40.6
50.8
61.0
3.What does the Bradford assay prevention? (1 point)
4.Which dye does the Bradford assay physical exercise? (1 point)
5.To determine the total protein content in a event fraction, what must you multiply by what? trace: there be 3 quantities! (3 points)
6.a. What dyes will we use for fluorescence microscopy & what do they stain? (4 points)
b. In the slides, the intensity of staining of _______________________________ is important. (1 point)
7.What is enzyme operation? (2 points)
8.What is the significance of total activity? (3 points)
9.What is the significance of specific activity? (3 points)
10.In what units will we measure enzyme activity? (2 points)
11.Which 2 enzymes are we following & where is to each one make? (4 points)
12.a.
With which instrument will we measure the amount of product form by the enzymatic reactions? (1 point)
b. What property must a message have in order to be measured by the instrument in part (a)? (1 point)
c. What is the measurable product of each enzyme reaction? (2 points)
13.For an enzymatic reaction, what should a blank contain? Hint: the answer is NOT “extra phosphate buffer”! (2 points)
14.What substances will you add to each enzyme reaction to stop it? What do these substances do? (5 points)
15.a. For succinate dehydrogenase, what volumes of enzyme go into the reactions? There are 2 different amounts, check to 2 different dilutions. (2 points)
b. If...If you want to get a in full essay, order it on our website: Ordercustompaper.com
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