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Lucigen Corporation 2905 Parmenter St, Middleton, WI 53562 USA doorbell Free: (888) 575-9695 | (608) 831-9011 | FAX: (608) 831-9012 lucigen@lucigen.com www.lucigen.com FOR RESEARCH USE ONLY. NOT FOR human OR DIAGNOSTIC USE
MA019 Rev. B, DCO No. 12-445
E. cloni EXPRESS Chemically competent Cells
Table of Contents
Comp atomic number 53nts & Storage Conditions ........................................................................................................ 2 E. cloni EXPRESS Chemically commensurate Cells ................................................................................... 3 Preparation for Transformation .............................................................................................................. 3 Transformation protocol ......................................................................................................................... 4 Media Recipes........................................................................................................................................ 5 Related Products .................................................................................................................................... 5
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Components & Storage Conditions
Three strains of Lucigens E.
cloni EXPRESS Chemically Competent Cells are available: BL21(DE3), BL21(DE3)pLysS, and BL21(DE3)pLysE. completely three strains of E. cloni EXPRESS Chemically Competent Cells have a transformation efficiency yield of > 1 x 107 cfu/µg pUC19. The cells are shipped on dry ice in one container, along with supercoiled control pUC19 DNA at 10 pg/µl and Expression Recovery Medium (lactose minus). E. cloni EXPRESS Chemically Competent Cells are available in 80 µl aliquots (DUOs), satisfactory for 2 transformations per tube. Please refer to the table below for catalogue numbers.
All E. cloni EXPRESS Competent Cells require storage at 80o C.
E. cloni ® EXPRESS Chemically Competent Cells:
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